HPLC Analysis of Amino Acids on Ascentis® Si (Silica), HILIC Mode

HPLC Analysis of Amino Acids on Ascentis® Si (Silica), HILIC Mode

Conditions

column Ascentis Si, 15 cm x 2.1 mm I.D., 5 µm particles (581509-U)
column temp. 35 °C
mobile phase [A] 100 mM ammonium formate (pH 3.0, with formic acid), [B] water, [C] acetonitrile
flow rate 0.3 mL/min
sample in 10:90, (50 mM ammonium formate/formic acid, pH 3.0):acetonitrile
injection 2 µL
detector ESI (+), full scan

Description

Categories Analytical Chromatography, Amino Acids
Featured Industry Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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