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HPLC Analysis of Amphetamines on Discovery® HS F5

HPLC Analysis of Amphetamines on Discovery® HS F5


column Discovery HS F5, 5 cm x 2.1 mm I.D., 3 µm particles (567500-U)
column temp. 35 C
mobile phase [A] 0.1% ammonium acetate in water; [B] 0.1% ammonium acetate in acetonitrile; [C] acetonitrile; (10:30:60, A:B:C)
flow rate 0.2 ml/min
sample 1 ug/mL in acetonitrile
injection 5 µl
detector MS, ESI (+) in Single Ion Recording (SIR) Mode


Categories Analytical Chromatography, Pharmaceutical Analysis, Amines
Featured Industry Forensics and Toxicology
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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