HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18

HPLC Analysis of Amphetamines in Urine on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and Standard C18

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix human urine spiked amphetamine and methylamphetamine at with 2 mg/mL
SPE tube/cartridge Discovery DSC-MCAX, 100 mg/3mL (52783-U)
condition DSC-MCAX SPE tube: 1 mL methanol; 1 mL 50 mM ammonium acetate, pH 6.0; C-18 SPE tube: 1 mL methanol; 1 mL DI water
sample addition 1 mL
washing DSC-MCAX SPE tube: 1 mL 50 mM ammonium acetate, pH 6.0; 1 mL 1M acetic acid; 1mL methanol; C-18 SPE tube: 1 mL DI water; 1 mL 20% methanol
elution DSC-MCAX SPE tube: 1 mL 5% ammonium hydroxide in methanol; C-18 SPE tube: 1 mL methanol
eluate post-treatment evaporate to dryness with nitrogen at room temperature, reconstitute in mobile phase
column Discovery HS F5, 15 cm x 4.6 mm I.D., 5 µm particles (567516-U)
column temp. 40 °C
mobile phase [A] 10 mM ammonium acetate, pH 4.5: [B] methanol; (35:65, A:B)
flow rate 2 mL/min
injection 10 µL
detector UV, 210 nm

Description

Application Sample Pre-Treatment:
1 mL human urine was spiked with 2 mg/mL amphetamine and methylamphetamine. The spiked sample was diluted 1:1 with 1:1 with 50 mM ammonium acetate, pH 6.0.
Categories Analytical Chromatography, SPE Applications, Forensics and Toxicology, drugs of abuse, Drugs of abuse, Pharmaceutical & Clinical applications, amines, ADHD drugs
Featured Industry Forensics and Toxicology
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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