HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

HPLC Analysis of an Energy Drink on Ascentis® Express HILIC (UV Detection)

Conditions

column Ascentis Express HILlC, 10 cm x 3.0 mm I.D., 2.7 µm particles (53970-U)
column temp. 35 °C
mobile phase [A] 100 mM ammonium acetate, pH 5.0 with acetic acid; [B] water; [C] acetonitrile; (09:01:90, A:B:C)
flow rate 0.6 mL/min
pressure 812 psi (56 bar)
sample Dilute 1:9 in acetonitrile
injection 2 µL
detector UV, 254 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of common drink additives used in caffeinated energy drinks. The analysis was performed with UVand ELSD detectors in series, this chromatogram being from the UV.
Categories Analytical Chromatography, Food & Beverage Analysis, Vitamins and Natural products, Beverage Testing, Carbohydrates, StiµLants, Organic Acids, Sports and Energy Drinks
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links