HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C8

HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C8

Conditions

column Discovery BIO Wide Pore C8, 15 cm x 4.6 mm I.D., 5 µm particles (568322-U)
column temp. 30 °C
mobile phase [A] 10 mM NH4H2PO4/ NH4OH, pH 7; [B] 50:50, (20 mM NH4H2PO4/ NH4OH, pH 7):CH3CN
gradient 30-60% B in 15 min
flow rate 1 mL/min
injection 6 µLin water
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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