Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C8

HPLC Analysis of Angiotensins on Discovery® BIO Wide Pore C8

Conditions

column Discovery BIO Wide Pore C8, 15 cm x 4.6 mm I.D., 5 µm particles (568322-U)
column temp. 30 C
mobile phase [A] 10 mM NH4H2PO4/ NH4OH, pH 7; [B] 50:50, (20 mM NH4H2PO4/ NH4OH, pH 7):CH3CN
gradient 30-60% B in 15 min
flow rate 1 ml/min
injection 6 muLin water
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links