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HPLC Analysis of Antibacterials/Antifungals on Ascentis® C18

HPLC Analysis of Antibacterials/Antifungals on Ascentis® C18


column Ascentis C18, 15 cm x 4.6 mm I.D., 5 µm particles (581324-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate (pH 3.0 with formicacid); [B] acetonitrile; (75:25, A:B)
flow rate 1.0 ml/min
sample 50 µg/mL each in acetonitrile
injection 5 µl
detector UV, 254 nm


Categories Analytical Chromatography, Pharmaceutical Analysis, Food & Beverage Analysis, Veterinary Drug Residues, Antibiotics, Antimicrobials, Antifungals, Applications (Chromatograms)
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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