HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® RP-Amide

HPLC Analysis of Antibiotic Sulfa Drugs on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 °C
mobile phase [A] 1% acetic acid in water; [B] methanol, (85:15, A:B)
flow rate 1.0 mL/min
sample as indicated in 1% acetic acid in water
injection 10 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Antibiotics, Antimicrobials, Antibiotics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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