HPLC Analysis of Aripiprazole and Dehydro Aripiprazole Using an Ascentis® Express C18 Column

HPLC Analysis of Aripiprazole and Dehydro Aripiprazole Using an Ascentis® Express C18 Column

Conditions

column Ascentis Express C18, 10 cm  x 3.0 I.D mm., 2.0 µm particles (50819-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium formate; pH 3.0 with formic acid [B] 0.1% formic acid in methanol
gradient 0 to 40% B in 0.5 min; to 95% B in 3.5 minutes; held at 95% B for 2 min
flow rate 0.5 ml/min
pressure 6500 psi (445 bar)
sample 50 µg/mL in 10% methanol
injection 2 µl
detector UV, 245 nm

Description

Analysis Note Aripiprazole is marketed as the atypical antipsychotic Abilify® for the treatment of schizophrenia, bipolar disorder and clinical depression. Dehydroapiprazole is the primary metabolite of Aripiprazole.
Categories Analytical Chromatography, Antidepressants
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Abilify is a registered trademark of Otsuka Pharmaceutical Co.
suitability application for HPLC

Materials

     
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