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HPLC Analysis of Artificial Food Colorants on Chromolith® HighResolution RP-18 endcapped, UV 628 nm

HPLC Analysis of Artificial Food Colorants on Chromolith® HighResolution RP-18 endcapped, UV 628 nm

Conditions

column Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 µm particles (1.52022.0001)
column temp. 30 C
mobile phase [A] 20mM ammonium acetate pH 6.8 in water; [B] methanol
gradient 5 to 50% B in 6 min; to 100% B in 4 min; held at 100% B for 1 min; to 5% B in 0.01 min
flow rate 1 ml/min
pressure 1526 psi (106 bar)
sample Brilliant Blue 0.05 mg/mL in methanol:water (50:50)
injection 20 µl
detector UV, 628 nm

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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