HPLC Analysis of Artificial Food Colorants in Dried Plums on Chromolith® HighResolution RP-18 endcapped

HPLC Analysis of Artificial Food Colorants in Dried Plums on Chromolith® HighResolution RP-18 endcapped

Conditions

column Chromolith HighResolution RP-18, 10 cm x 4.6 mm I.D., 2 mu particles (1.52022.0001)
column temp. 30 C
mobile phase [A] 20mM ammonium acetate pH 6.8 in water; [B] methanol
gradient 5 to 50% B in 6 min; to 100% B in 4 min; held at 100% B for 1 min; to 5% B in 0.01 min
flow rate 1 ml/min
pressure 1526 psi (106 bar)
injection 20 µl
detector UV, 510 nm

Description

Categories Analytical Chromatography, Dyes
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes 5 g of powdered dried fruit was transferred to a 50 mL volumetric flask. 45 mL of diluent was added into the flask. The flask was shaken for 30 seconds, then sonicated for 10 minutes. Diluent was added up to volume after the flask was cold. The solution was transferred to a 50 mL PP tube, centrifuged at 4000 rpm for 10 minutes, thereafter the clear solution was filtered by 0.45 μm PTFE syringe filter into a 2 mL autosampler vial prior to injection.
suitability application for HPLC

Materials

     
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