Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide

HPLC Analysis of Ascorbic Acid on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase 25 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid)
flow rate 1 ml/min
sample as indicated each in mobile phase
injection 10 µl
detector UV, 230 nm

Description

Categories Analytical Chromatography, Organic Acids
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links