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HPLC Analysis of Ascorbic Acid in White Wine: Scaling Separations from Analytical to Semi-Preparative on SeQuant ZIC-HILIC, 4.6 mm to 20 mm I.D. Columns

HPLC Analysis of Ascorbic Acid in White Wine: Scaling Separations from Analytical to Semi-Preparative on SeQuant ZIC-HILIC, 4.6 mm to 20 mm I.D. Columns

Conditions

column SeQuant ZIC-HILIC, SS, 150 x 4.6 mm or 20 mm, 5 μm, 200 Å (1.50497.0001, 1.50455.0001)
mobile phase [A] 0.1% (v/v) formic acid; [B] acetonitrile; (25:75, A:B)
flow rate 20 ml/min
flow rate 1.0 ml/min
pressure 720 psi (5.0 MPa)
injection 250 and 5000 µl of white wine diluted 1:3 with acetonitrile
detector 240 nm

Description

Featured Industry Food and Beverages
suitability application for HPLC

Materials

     
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