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HPLC Analysis of Aswagandha Extract on Discovery® C18

HPLC Analysis of Aswagandha Extract on Discovery® C18


column Discovery C18, 25 cm x 4.6 mm I.D., 5 µm particles (504971)
column temp. 27 C
mobile phase [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate 1.5 ml/min
pressure 3191 psi (220 bar)
sample 20 µg/mL in water: acetonitrile (86:14)
injection 20 µl
detector UV, 227 nm


Analysis Note This application demonstrates the suitability of Discovery C18 for the efficient separation of withania extract by the USP monograph.
Categories Analytical Chromatography, Herbal medicines
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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