HPLC Analysis of Atrazine Herbicides on Ascentis® Express C18

HPLC Analysis of Atrazine Herbicides on Ascentis® Express C18

Conditions

column Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 µm particles (53814-U)
column temp. 46 C
mobile phase [A] 20 mM ammonium acetate, pH 6.4 unadjusted; [B] acetonitrile
gradient 20 to 28% B in 11 min; 28 to 65% B in 5 min; held at 65% B for 4 min
flow rate 0.6 ml/min
sample 10-25 µg/mL in 10:90, water: acetonitrile
injection 5 µl
detector UV, 240 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 17 triazine pesticides.
Categories Analytical Chromatography, Pesticides & Herbicides
Featured Industry Agriculture
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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