HPLC Analysis of Azithromycin on Ascentis® Express C18 5μm

HPLC Analysis of Azithromycin on Ascentis® Express C18 5μm

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 5 µm particles (50537-U)
column temp. 45 °C
mobile phase [A] 10 mM potassium phosphate, dibasic, pH 7.0 with phosphoric   acid; [B] methanol; (20:80, A:B)
flow rate 1.5 mL/min
pressure 2558 psi (176 bar)
injection 10 µL
detector UV, 210 nm

Description

Analysis Note A simple high-performance liquid chromatography (HPLC) method was developed for the determination of azithromycin in raw materials and pharmaceutical forµLations without any pretreatment or derivatization step.
Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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