HPLC Analysis of B-vitamins on Ascentis® Express RP-Amide using UV detection

HPLC Analysis of B-vitamins on Ascentis® Express RP-Amide using UV detection

Conditions

column Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 µm particles (53918-U)
column temp. 30 °C
mobile phase [A] 20 mM potassium phosphate, pH 3; [B] methanol;
gradient 0.5% B held for 1.5 minutes, to 25% B in 1.0 minutes, held at 25% B for 1.7 minute, held at 0.5% B for 1.2 minutes
flow rate 0.75 mL/min
pressure 5584 psi (385 bar)
sample 100 µg/mL of all vitamins except 250 µg/mL of calcium pantothenate, biotin and riboflavin in water
injection 0.5 µL
detector UV, 210 nm

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Vitamins and Natural products, Anticonvulsant/antiepiletic/antiseizure
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for UHPLC

Materials

     
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