HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase

HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase

Conditions

column Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 µm particles (53348-U)
column temp. 35 C
mobile phase [A] water; [B] acetonitrile; (62:38, A:B)
flow rate 1.4 ml/min
pressure 2600 psi (179 bar)
sample 0.1 g/mL of each in 20% methanol
injection 2 µl
detector UV, 250 nm

Description

Analysis Note The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
Categories Analytical Chromatography, Pharmaceutical Analysis, Clinical, antipsychotics, antiepileptic/antiseizure, Anticonvulsant/antiepiletic/antiseizure, Antipsychotics
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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