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HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide ("Green" Mobile Phase Conditions)

HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide (

Conditions

column Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 µm particles (53916-U)
column temp. 55 C
mobile phase [A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)
flow rate 0.4 ml/min
pressure 138 bar (2000 psi) column, 248 bar (3600 psi) total
sample 50 µg/mL in mobile phase
injection 1 µl
detector UV, 215 nm

Description

Categories Analytical Chromatography, Organic Acids
Featured Industry Cosmetics, Personal Care, and Cleaning Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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