Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Beta Blockers on Ascentis® Si

HPLC Analysis of Beta Blockers on Ascentis® Si

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 35 C
mobile phase [A] 0.1% ammonium acetate in water (pH unadjusted); [B] 0.1% ammonium acetate in acetontrile; (15:85, A:B)
flow rate 1 ml/min
sample as indicated in 0.1% ammonium acetate in 10:90:water:acetonitrile
injection 10 µl
detector UV, 225 nm

Description

Categories Analytical Chromatography, Beta-blockers
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links