HPLC Analysis of Bile Acids on Ascentis® Express C18

HPLC Analysis of Bile Acids on Ascentis® Express C18

Conditions

column Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 µm particles (53826-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium acetate, pH 4 (adjusted with acetic acid); [B] acetonitrile; (80:20, A:B)
gradient 20 to 100% B in 10 min
flow rate 1.0 ml/min
sample 10 µg/mL
injection 5 µl
detector ESI(-) in SIR mode

Description

Categories Analytical Chromatography, Clinical, Organic Acids, organic acids
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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