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HPLC Analysis of Bilirubin and Biliverdin on Discovery® C18

HPLC Analysis of Bilirubin and Biliverdin on Discovery® C18

Conditions

column Discovery C18, 10 cm x 2.1 mm I.D., 5 µm particles (569220-U)
column temp. ambient
mobile phase [A] 10 mM ammonium acetate (pH unadjusted) [B] 90:10, methanol:(100 mM ammonium acetate)
flow rate 0.2 ml/min
sample as indicated (in 50% MeOH, 1% NH4OH)
injection 1 µl
detector UV, 405 nm

Description

Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, Antioxidants, antioxidants, Proteins
Featured Industry Clinical
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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