HPLC Analysis of Biotin Enantiomers on Astec® CHIROBIOTIC® T

HPLC Analysis of Biotin Enantiomers on Astec® CHIROBIOTIC® T

Conditions

column CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 µm particles (12024AST)
column temp. 23 °C
mobile phase [A] 0.1% sodium phosphate, pH 4.1; [B] methanol; (60:40, A:B)
flow rate 1 mL/min
sample 4 mg/mL in methanol
injection 2 µL
detector UV, 210 nm

Description

Categories Analytical Chromatography, Chiral Applications, Life Science & Biopharma, Vitamins and Natural products, Food & Beverage, All Food & Beverage Applications, All Biochemical Applications, Vitamins & Coenzymes, Vitamins
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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