HPLC Analysis of BPA (Bisphenol A) at 1 ng/mL in Milk on Ascentis® Express C18 after Solid Phase Extraction using SupelMIP® SPE - Bisphenol A

HPLC Analysis of BPA (Bisphenol A) at 1 ng/mL in Milk on Ascentis® Express C18 after Solid Phase Extraction using SupelMIP® SPE - Bisphenol A

Conditions

sample preparation (reconstitute residue in 500 µL 40:60 acetonitrile;water)
sample preparation SPE (Solid Phase Extraction)
SPE tube/cartridge SupelMIP SPE - Bisphenol A, 100 mg/6 mL (54277-U)
condition 5 mL methanol 2% acetic acid, 5 mL acetonitrile, 5 mL water (1 drop/sec)
sample addition 5 mL liquid milk, 1 drop/2 sec
washing 7 mL water followed by 4 mL 40:60 acetonitrile;water
drying -12" vacuum for 120 sec post wash
elution 2 mL methanol
eluate post-treatment evaporate to dryness at 45 °C under 10 psi nitrogen stream
column Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 µm particles
column temp. 30 °C
mobile phase [A] acetonitrile; [B] water
gradient 40% A held for 3.5 min; to 60% A in 0.1 min; held for 3 min; to 40% A in 0.1 min; held for 13.3 min
flow rate 0.20 mL/min
injection 5.0 µL
detector FLD, ex 230 nm; em 315 nm

Description

Analysis Note Bisphenol A (BPA) is a commonly used synthetic chemical added to a wide variety of plastics and epoxy resins to improve the stability of the final product. BPA has been under scrutiny as an endocrine disruptor, so its level in consumer products, food, and environmental and biological systems is an area of active research and regulation. This application shows a means to purify, concentrate, analyze, and quantify BPA in liquid milk to 1 ng/mL (1 ppb) using SPE and HPLC with fluorescence detection. SupelMIP® SPE - Bisphenol A was used to extract the compound from the milk matrix. Analysis was via HPLC on an Ascentis® Express C18. High purity LC-MS solvents used in the mobile phase provided robust, clean operation.
Categories Analytical Chromatography, Food & Beverage Analysis, Phenols, Beverage Testing, Milk
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, SupelMIP is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SPE, application for HPLC

Materials

     
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