HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

Conditions

column TSKgel Bio Assist S, 5 cm x 4.6 mm I.D., 7 µm particles (819686)
column Competitive S-type column, 5 cm x 5 mm I.D.
column temp. 25 °C
mobile phase [A] 20 mM sodium phophate buffer, pH 7.0; [B] 20 mM sodium phophate buffer, 0.5 M NaCl, pH 7.0
gradient 0 to 100% B in 20 min (0 to 100% B in 30 min for competitive column)
flow rate 0.8 mL/min (1.0 mL/min for competitive column)
sample crude bromelain, 0.01 µg/mL
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information BioAssist is a registered trademark of Tosoh Corporation, TSKgel is a registered trademark of Tosoh Corporation
Other Notes Data provided by Tosoh Bioscience LLC.
suitability application for HPLC

Materials

     
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