HPLC Analysis of Caffeine and Metabolites on Ascentis® RP-Amide

HPLC Analysis of Caffeine and Metabolites on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 30 °C
mobile phase [A] 0.1% ammonium acetate in water, pH 4.5 with acetic acid; [B] acetonitrile
gradient 2 to 8% B in 3 min; to 20% B in 9 min; held for 2 min; to 2% B in 0.1 min; held for 0.9 min
flow rate 1.0 mL/min
sample as indicated in 0.1% ammonium acetate in water (pH 4.5 with acetic acid)
injection 10 µL
detector UV, 275 nm

Description

Categories Analytical Chromatography, Clinical, stiµLants, StiµLants, drugs of abuse, Drugs of Abuse
Featured Industry Clinical
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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