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HPLC Analysis of Caffeine, Choline, Taurine, Inositol, and Carnitine on SeQuant ZIC-HILIC

HPLC Analysis of Caffeine, Choline, Taurine, Inositol, and Carnitine on SeQuant ZIC-HILIC

Conditions

column Sequant ZIC-HILIC (3.5 µm, 100 Ã…) PEEK, 150 x 2.1 mm (1.50442.0001)
column temp. 30 C
mobile phase [A] acetonitrile; [B] 100 mM ammonium acetate, pH 4.5
gradient 100% A held for 2 min; to 40% A in 10 min; to 100% A in 5 min
flow rate 0.4 ml/min
injection 5 µl
detector ELSD (Sedere Sedex 85LT), 40C, 3,5 bar pressurized air, gain 6

Description

Featured Industry Food and Beverages
suitability application for HPLC

Materials

     
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