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HPLC Analysis of Calphostin C on Discovery® HS C18

HPLC Analysis of Calphostin C on Discovery® HS C18


column Discovery HS C18, 25 cm x 2.1 mm I.D., 5 µm particles (568503-U)
column temp. 24 C
mobile phase [A] (0.1% phosphorus acid in water):(0.1% phosphorus acid in acetonitrile); [B] 0.1% phosphorus acid in acetonitrile; (50:50, A:B)
flow rate 0.2 ml/min
sample Calphostin C (0.25 g/L) in 40% MeOH, 0.025% TFA
injection 3 µl
detector UV, 254 nm


Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, antibiotics/antimicrobials, Antibiotics
Featured Industry Clinical
Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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