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HPLC Analysis of Cannabinoids on Ascentis® C18

HPLC Analysis of Cannabinoids on Ascentis® C18


column Ascentis C18, 10 cm x 4.6 mm I.D., 3 µm particles (581321-U)
column temp. 35 C
mobile phase [A] 10 mM ammonium acetate (pH 4.5 with acetic acid); [B] acetonitrile; (25:75, A:B)
flow rate 1 ml/min
sample 30 µg/mL each in mobile phase
injection 10 µl
detector UV, 220 nm


Categories Analytical Chromatography, Forensics and Toxicology, drugs of abuse, analgesics, Analgesics
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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