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HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18

HPLC Analysis of Carboxymethylated Apohemoglobin (Horse) Tryptic Digest on Discovery® BIO Wide Pore C18

Conditions

column Discovery BIO Wide Pore C18, 15 cm x 2.1 mm I.D., 3 µm particles (568222-U)
column temp. 35C
mobile phase [A] 0.1% TFA in water; [B] 0.1% TFA in water:0.1% TFA in acetonitrile, 50:50;
gradient 0-100% B in 50 min
flow rate 0.2 ml/min
injection 5 µL, each at 0.8 mg/mL in 0.1% TFA
detector UV, 220 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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