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HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C5

HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C5

Conditions

column Discovery BIO Wide Pore C5, 15 cm x 4.6 mm I.D., 5 µm particles (568422-U)
column temp. 30 C
mobile phase [A] 0.1% TFA in water:0.1% TFA in acetonitrile, 95:5; [B] 0.1% TFA in water:0.1% TFA in acetonitrile; 50:50
gradient 0-100% B in 65 min
flow rate 1 ml/min
sample carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
injection 50 µl
detector UV, 215 nm

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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