HPLC Analysis of Carotenes on Ascentis® Express RP-Amide, Methanol Mobile Phase

HPLC Analysis of Carotenes on Ascentis® Express RP-Amide, Methanol Mobile Phase

Conditions

column Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 µm particles (53931-U)
column temp. 30 °C
mobile phase methanol
flow rate 1 mL/min
pressure 1653 psi (114 bar)
sample V8 Splash® fruit juice was extracted with hexane, the extract dissolved 1:1 into ethanol for HPLC injection
injection 5 µL
detector UV-VIS, 454 nm

Description

Analysis Note Analysis was conducted using juice extract, detection via UV.
Categories Analytical Chromatography, Food & Beverage Analysis, Pigments, Vitamins and Natural products, Beverage Testing, Hydrocarbons, Fruit Juice
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, V8 Splash is a registered trademark of CSC Brands, LP
suitability application for HPLC

Materials

     
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