HPLC Analysis of Carotenoids on Ascentis® Si (Silica), Normal Phase

HPLC Analysis of Carotenoids on Ascentis® Si (Silica), Normal Phase

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 30 °C
mobile phase [A] hexane: [B] ethyl acetate: [C] isopropanol; (85:10:5, A:B:C)
flow rate 1.0 mL/min
sample as indicated in hexane
injection 10 µL
detector UV-VIS, 450 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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