HPLC Analysis of Caseins on BIOshell™ A400 Protein C4

HPLC Analysis of Caseins on BIOshell™ A400 Protein C4

Conditions

column BIOshell A400 Protein C4, 10 cm x 2.1 mm I. D., 3.4 µm particles (66825-U)
column temp. 50 C
mobile phase [A] 80:20 Water (0.1% trifluoroacetic acid): Acetonitrile (0.1% trifluoroacetic acid); [B] 20:80 Water (0.1% trifluoroacetic acid): Acetonitrile (0.1% trifluoroacetic acid)
gradient 20 to 53% B in 5 min
flow rate 0.3 ml/min
pressure 1150 psi (79 bar)
sample Three component casein mix, 200 µg/mL, 0.1% trifluoroacetic acid
injection 10 µl
detector UV, 215 nm

Description

Analysis Note Caseins are a group of phosphoproteins found predominantly in mammalian milk. Each type of mammalian milk has a different profile of caseins (alpha, beta, etc.) based on the mammal′s diet and where it is located. Therefore, this profile can be used to authenticate where certain dairy products (i.e. cheeses) came from. A RP-HPLC method for the separation of three caseins (alpha, beta, and kappa-casein) was developed on the BIOshell A400 Protein C4 column.
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
Other Notes Two isoforms of beta-casein exist in mammalian milk: A1-beta-casein and A2-beta-casein; therefore, two peaks are identified for beta-casein., Due to the heterogeneity of the standards, band broadening and extra peaks resulted in the chromatogram.
suitability application for HPLC

Materials

     
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