HPLC Analysis of Catecholamines on Discovery® C18

HPLC Analysis of Catecholamines on Discovery® C18

Conditions

column Discovery C18, 15 cm x 4.6 mm I.D., 5 µm particles (504955)
column temp. 20 °C
mobile phase [A] acetonitrile: [B] 50 mM KH2PO4, pH 3.0 with phosphoric acid, 100 mg/L EDTA, 200 mg/L 1-octane-sulfonic acid (5:95, A:B)
flow rate 1 mL/min
injection 10 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Hormones
Featured Industry Pharmaceutical (small molecule)
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links