HPLC Analysis of Cetuximab Antibody Fragments from Papain Digestion on Zenix® SEC-150

HPLC Analysis of Cetuximab Antibody Fragments from Papain Digestion on Zenix® SEC-150

Conditions

column Zenix SEC-150, 30 cm x 7.8 mm I.D., 3 µm, 150 Ã… particles (Z777018)
column temp. 25 C
mobile phase 20 mM sodium phosphate, pH 7.0, 150 mM NaCl
flow rate 1.0 ml/min
sample Cetuximab ERBITUX(R), papain digest
injection 10 µl
detector UV, 280 nm

Description

Categories Analytical Chromatography, Antibodies
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies, Erbitux is a registered trademark of Eli Lilly and Co.
Other Notes Papain digestion conditions: Buffer: 5.0 mM L-Cysteine, 2.0 mM EDTA and 300 mM NaCl; Enzyme: 80 μg/mL papain; Temperature: 37 °C; Time: 30 min, 60 min, 120 min, 240 min, and 360 min.
suitability application for HPLC

Materials

     
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