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HPLC Analysis of Codeine and Metabolite on Ascentis® RP-Amide

HPLC Analysis of Codeine and Metabolite on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetonitrile; (90:10, A:B)
flow rate 1 ml/min
sample 50 µg/mL each in mobile phase
injection 10 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Clinical, Forensics and Toxicology, Pharmaceutical Analysis, analgesics, Analgesics
Featured Industry Clinical
Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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