HPLC Analysis of Conjugated Flavonoids on Discovery® HS-C18

HPLC Analysis of Conjugated Flavonoids on Discovery® HS-C18

Conditions

column Discovery HS C18, 15 cm x 4.6 mm I.D., 3 µm particles (569252-U)
column temp. 60 °C
mobile phase [A] 10 mM sodium citrate (pH 7.0 with 10 mM citric acid); [B] acetonitrile; (80:20, A:B)
flow rate 1 mL/min
sample 50 µg/mL each (Maysin, Apimaysin, Methoxymaysin) in 10 mM sodium citrate (pH 7.0 with 10 mM citric acid)
injection 10 µL
detector UV, 340 nm

Description

Categories Analytical Chromatography, Antioxidants
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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