HPLC Analysis of Cosmetic Preservatives on Ascentis® Express RP-Amide

HPLC Analysis of Cosmetic Preservatives on Ascentis® Express RP-Amide

Conditions

column Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 µm particles (53918-U)
column temp. 50 C
mobile phase [A] water; [B] methanol; (60:40, A:B)
flow rate 0.6 ml/min
pressure 2900 psi (200 bar)
sample methyl-, benzylparaben (50 mg/L), ethylparaben (30 mg/L), propyl-, butylparaben (100 mg/L), 2-phenoxyethanol (1.5 g/L),1-phenoxy-2-propanol (3 g/L), all in 40% methanol
injection 1 µl
detector UV, 250 nm

Description

Analysis Note With the publication Borremans, M. et.al. 2004, Chromatographia 59: 47-53 as a reference, this method is 10% faster, but with superior resolution of critical pairs; in this case, the miniµm USP resolution is 2.4.
Categories Analytical Chromatography, Preservatives
Featured Industry Cosmetics, Personal Care, and Cleaning Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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