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HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

Conditions

column Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 µm particles (59601-U)
column temp. 25 C
mobile phase [A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)
flow rate 0.5 ml/min
injection 10 mug each variant
detector UV, 280 nm

Description

Analysis Note This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.
Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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