HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX

Conditions

column Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm I.D., 5 µm particles (56901-U)
mobile phase [A] 20 mM Bis-Tris HCl, pH 7.0; [B] A + 0.5 M NaCl 24-69% B over 20 min
flow rate 0.5 mL/min
injection 10 mugeach variant
detector UV, 280 nm

Description

Categories Analytical Chromatography, Proteins
Featured Industry Life Science and Biopharma
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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