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HPLC Analysis of Disaccharides Turanose, Lactulose, and Melibiose on Chromolith® NH2

HPLC Analysis of Disaccharides Turanose, Lactulose, and Melibiose on Chromolith® NH2

Conditions

column Chromolith NH2, 10 cm x 4.6 mm I.D., 2 µm particles (1.52028.0001)
column temp. 23 C
mobile phase [A] acetonitrile; [B] water; (85:15, A:B)
flow rate 4 ml/min
pressure 1102 psi (76 bar)
sample turanose 7.7 mg/mL, lactulose 8.0 mg/mL, melibiose 10.6 mg/mL in mobile phase
injection 10 µl
detector UV, 190 nm

Description

Categories Analytical Chromatography, Carbohydrates
Featured Industry Food and Beverages
Legal Information Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
suitability application for HPLC

Materials

     
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