HPLC Analysis of Diuretic Drugs on Ascentis® RP-Amide

HPLC Analysis of Diuretic Drugs on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] 10 mM monobasic potassium phosphate (pH 3.0 with phosphoric acid); [B] acetronitrile; (60:40, A:B)
flow rate 1 ml/min
sample 50 µg/mL each in mobile phase
injection 10 µl
detector UV, 234 nm

Description

Categories Analytical Chromatography, Diuretics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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