HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

Conditions

column Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53569-U)
column temp. 35 °C
mobile phase [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water
gradient held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min
flow rate 0.3 mL/min
pressure 175 bar
sample 1 µg/mL in 50:50, water: methanol
injection 1 µL
detector ESI(+), m/z 100-1000

Description

Categories Analytical Chromatography, Forensics and Toxicology, Drugs of Abuse, drugs of abuse
Featured Industry Forensics and Toxicology
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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