HPLC Analysis of Exenatide Acetate on Proteomix® SCX-NP5

HPLC Analysis of Exenatide Acetate on Proteomix® SCX-NP5

Conditions

column Proteomix SCX NP5, 25 cm x 4.6mm I.D., 5 µm particles (Z777161)
column temp. 40 C
mobile phase (A) 10 mM monosodium phosphate:acetonitrile (56:44, v/v), adjusted to pH to 2.0 with phosphoric acid; ((B) 0.5 M NaCl/10 mM monosodium phosphate:acetonitrile (56:44, v/v), adjusted to pH to 2.0 with phosphoric acid)
gradient 0 to 65% B in 35 min; held for 5 min; to 0% B in 10 min
flow rate 1.0 ml/min
pressure 1653 psi (114 bar)
sample 0.325 mg/mL exenatide acetate in mobile phase A
injection 20 µl
detector UV, 235 nm

Description

Categories Analytical Chromatography, Antihyperglycemic
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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