HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column

Conditions

column Ascentis Si, 15 cm x 4.6 mm I.D., 5 µm particles (581512-U)
column temp. 30 C
mobile phase [A] hexane, [B] ethyl acetate
gradient 10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min
flow rate 1.0 ml/min
sample as indicated in 96:4 (hexane:isopropanol)
injection 10 µl
detector UV, 290 nm

Description

Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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