HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide

HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 °C
mobile phase acetonitrile
flow rate 2.0 mL/min
injection 20 µL
detector UV, 215 nm

Description

Analysis Note The Ascentis RP-Amide is shown to be suitable for the analysis of fat soluble vitamins, alpha tocopherol (vitamin E) and cholecalciferol (vitamin D3 ).
Categories Analytical Chromatography, Vitamins and Natural products
Featured Industry Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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