HPLC Analysis of Fatty Acids on Ascentis® RP-Amide

HPLC Analysis of Fatty Acids on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 µm particles (565324-U)
column temp. 35 C
mobile phase [A] 0.1% formic acid in water: [B] acetonitrile; (5:95, A;B)
flow rate 1.0 ml/min., split to detector
sample 10 µg/mL in acetonitrile
injection 5 µl
detector ESI(-)

Description

Categories Analytical Chromatography, Fatty Acids, FAMEs
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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