HPLC Analysis of Flavonoids in Orange Juice on Ascentis® C18

HPLC Analysis of Flavonoids in Orange Juice on Ascentis® C18

Conditions

column Ascentis C18, 25 cm x 4.6 mm I.D., 5 µm particles (581325-U)
column temp. 25 °C
mobile phase [A] formic acid: [B] 2-propanol: [C] acetonitrile: [D] water (0.1:10:12:78, A:B:C:D:)
flow rate 0.8 mL/min
sample orange juice, passed through a 0.45 µm filter
injection 10 µL
detector PDA, 283 nm

Description

Categories Analytical Chromatography, Food & Beverage Analysis, Flavors, Fragrances, Beverage Testing, Fruit Juice
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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