HPLC Analysis of Fluorinated Pyrimidine Nucleosides on Ascentis® RP-Amide

HPLC Analysis of Fluorinated Pyrimidine Nucleosides on Ascentis® RP-Amide

Conditions

column Ascentis RP-Amide, 5 cm x 4.6 mm I.D., 5 µm particles (565323-U)
column temp. 30 °C
mobile phase [A] water with 0.1% ammonium formate (pH 3.04 with formic acid), [B] acetonitrile
flow rate 1.0 mL/min
sample as indicated in mobile phase A
injection 5 µL
detector UV, 260 nm

Description

Categories Analytical Chromatography, Clinical, nucleic acids, Nucleic Acids
Featured Industry Clinical
Life Science and Biopharma
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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