Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Phenyl

HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Phenyl

Conditions

column Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 µm particles (581616-U)
column temp. 35 C
mobile phase [A] 13 mM ammonium formate (pH 3.0 with concentrated formic acid): [B] acetonitrile; (80:20, A:B)
flow rate 1 ml/min, split to the MS
sample as indicated in 80:20, 13 mM ammonium formate (pH 3.0 with concentrated formic acid):acetonitrile
injection 5 µl
detector MS, ESI (+) in selected ion recording (SIR) mode

Description

Categories Analytical Chromatography, Pharmaceutical Analysis, Antibiotics, Antibiotics, Antimicrobials
Featured Industry Food and Beverages
Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
Related Links